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Individual Cq values were considered as valid only if the amplification parameters passed all quality checks.
The amplification parameters were same as above.
The amplification parameters were initiated at 95°C for 5 min, then 95° C for 30 s, 53°C for 30 s, and 72°C for 30 s for 35 cycles, followed by 7 min at 72°C for the final extension.
The temperature switch PCR (TSP) [ 17] strategy was adopted to optimize the amplification parameters.
The amplification parameters were 1 min at 95°C, 1 min at 62°C and 1 min at 72°C for 28 cycles.
The amplification parameters were 40 cycles of two steps, each step comprised of heating at 95°C and extension at 60°C.
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There are many choices for the selection of the amplification parameter α in relaying systems[5, 20].
Riihonen et al.[5] demodulate the OFDM symbols at the RS to amplify each subcarrier separately with the amplification parameter α.
Small uncertainties in the measured temperatures can, however, create large uncertainties in the calculated value of h, and the amplification parameter, Φh, is defined as the ratio of the relative uncertainty in h to the relative uncertainties in the temperatures.
The amplification parameter α 1 j l = P R 1 E | Y R 1 j l | 2 for the j th antenna on the l th subcarrier is again selected such that the total transmitted power at R1 is P R 1. Before transmitting the OFDM signal onto the next hop, it is passed through the HPA at the RS.
The PCR amplification parameters recommended by the manufacturer were used.
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