Exact(7)
In both cases, FEM values could be associated with experimental results, by direct measurement of pileup heights and by the amount of circular cracks that propagated during single indentations, respectively.
We apply supervised feature selection to limit the amount of circular fingerprint fragments as an alternative to folding.
Upon isolation of total DNA, it should be possible to simultaneously quantify the recombination efficiency and the amount of circular excised fragment using eMLPA.
By packaging the viral vector with the inactive D64V integrase mutant we were able to increase the amount of circular substrates 4-fold and at the same time abolish the viral integration machinery.
The final construct was dissolved in 1 mM Tris-HCl and 0.1 mM EDTA (pH 8), and a portion was subjected to electrophoresis on a 1% agarose gel to assess the amount of circular DNA.
The final construct was dissolved in 1 mM Tris-HCl-0.1 mM EDTA, pH 8, and a portion was subjected to electrophoresis on 1% agarose gel in order to assess the amount of circular DNA.
Similar(53)
Therefore, 'percent supercoiling' reflects the amount of closed circular plasmid DNA that is packaged into chromatin and does not include the nicked DNA that is packaged into chromatin.
For each well of a 24-well-plate, 2×10 cells were seeded one day before transfection, transfected with 0.8 μg of circular PB[BAC] and 0.2 μg of circular CMV-PBase DNA as the test group, or the same amount of circular PB[BAC] with 0.2 μg of circular pcDNA4/HisA as the control.
In multiple independent experiments, the mice injected with L-PAC-transfected parasites developed an parasitemia of ∼1% 6 days after transfection, whereas a parasitemia of ∼1% level was not observed until day 7 or 8 in mice injected with parasites transfected with the same amount of circular plasmid DNA, i.e., the C-PAC or pbCEN5A/T.
In their study, they have estimated only the relative amount of circular mtDNA molecules with deletions between genotypes.
To model this response it was assumed the serotonin release occurred instantaneously, had a long decay and was directly proportional to the amount of activity in the circular muscle.
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