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DOI: http://dx.doi.org/10.7554/eLife.05663.009 To put these numbers in context and gain a deeper understanding on how the amount, chain-length, and 2-methylation of hopanoids impact membrane biophysical properties, we performed membrane rigidity measurements.

It was found that the branching reaction may take place in any protein fraction in native CPI; furthermore, by increasing the amount of chain extender, more native protein chains in CPI were coupled to form branches.

The results suggest that the glucuronyl transferase (PmHS2-GlcUA+) is the limiting catalyst in the overall PmHS2 polymerization process, either by controlling the amount of chain initiated and by exhibiting per heparosan chain a slow and a constant rate in transferring GlcUA residue in comparison to the GlcNAc transfer by PmHS2-GlcNAc+.

Bulk copolymer composition is independent of the amount of chain transfer agent in the higher MW range ( > 20 000).

Bulk copolymer composition is independent of the amount of chain transfer.

When the molecular weight of the soft segment is 900, and the amount of chain extender is reduced by 40%, the molecular size is 750 nm.

Such changes in viscoelastic response were consequences of increase in the amount of chain entanglements additionally formed by the linear blocks.

20 Additionaly, effective sample size (ESS) is also reported to indicate the amount of chain mixing.

However, in ss1/be1, the amount of chains with DP 7 and DP 18 was higher, and the amount of chains with DP 9 was lower, compared with those in the calculated profile.

The amount of chains initiated will not differ depending on the nucleotide sugar concentrations, and thus, low substrate concentrations will result in shorter polysaccharides.

When these distributions are displayed as ln(number molecular weight distribution), readily obtained from the GPC trace (essentially by dividing by the square of molecular weight), the results can be semi-quantitatively interpreted in terms of the amounts of chain stoppage by transfer and by termination.