Exact(5)
For the altitude classes, more marked differences were seen (Table 1).
For the altitude classes, the situation was different; indeed, all of the SSR loci were significantly differentiated among the altitude classes, with values varying from 0.03 (Bmag0013) to 0.28 (HVM67).
The T3 cluster was mostly present at intermediate and high altitudes, while T4 was present in all of the altitude classes.
Among the altitude classes, the levels of differentiation were higher compared to seasons and districts, both for FST (0.10, P < 0.001) and RST (0.09, P < 0.001).
Finally, compared to seasons and districts, the altitude classes had a higher number of private alleles (17; 26%), four of which (one for <2,300 m a.s.l., and three for >2,800 m a.s.l).l
Similar(55)
The differentiation among the low altitude classes of the three districts was higher (FST = 0.10, P < 0.01) than that among the three districts within the intermediate (FST = 0.04, P > 0.05) and high (FST = 0.01, P > 0.05) altitude classes.
Averaging the q for the six TESS clusters for the three altitude classes, we observed that the previous cline of variation along altitude identified by the STRUCTURE assignment was also maintained for TESS; indeed, at low altitudes, the T1 and T2 clusters were mainly present, and these tended to be substituted by the T5 and T6 clusters at high altitudes.
Table 4 reports the divergence estimates (FST) among the three districts within the same altitude classes (<2,300 m a.s.l.; 2,300-2,800 m a.s.l., and >2,800 m a.s.l).l
These were analysed as averages for each of the seasons (Belg and Meher), the districts (Ankober, Mojanawadera, Tarmaber), and the three altitude classes (<2,300, 2,300-2,800, >2,800 m a.s.l)., and for the whole sample, using the POPGENE software, version 1.31 [ 56].
Table 1 provides a summary of the statistics computed considering each of the two seasons (Belg and Meher), the three districts (Ankober, Mojanawadera, and Tarmaber) and the three altitude classes (<2,300, 2,300-2,800, >2,800 m a.s.l).l
To include the cable crane lines in one or other of the two operational altitude classes, the mean altitude was considered, calculated as the altitude of the downhill anchor point plus the semi-difference between the altitudes of the two anchor points.
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