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Resulting sequences are clustered and aligned, and the alignments are mined for SNPs using custom-developed bio-informatics tools.
This description should make it clear that the alignments are reshuffled over the entire aligned region encompassing all of the domains.
This process was performed using a web interface tool described in the alignments procedures section of the methods, where the data on the alignments are reported and graphically displayed.
Of the 186 TEI 171 could be aligned to 3 or more reads and most of the alignments are highly consistent in their breakpoint position, with variation close to null.
The structural alignments of the 8 subunits (4 MEC-4 and 4 UNC-8) were aligned in alternating order to the superimposed chains of 2QTS and the alignments are submitted separately to the SWISS-MODEL server (http://swissmodel.expasy.org/).org/
Sequences in functional families are aligned using MAFFT [33] and conservation scores for each position in the alignments are computed using Scorecons [34].
Often the alignments are surprising.
The alignments are a product of ideology, not biology.
Therefore, one must be careful to perform adequate statistical tests to make sure the alignments are significant and not just accidental.
The alignments are similar at both ends for the two optimization methods, whereas the middle sections of the alignments are shifted to connect different selected stations.
Once the alignments are defined, the road site can be divided into homogeneous segments.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com