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Ambiguously aligned or highly diverged regions of the alignment were masked with three different algorithms [VII].
Poorly aligned and gap-rich regions of the alignment were identified by eye and excluded from the analyses.
Thus, the poorly aligned positions, gap positions and divergent regions from the alignment were completely excluded from the phylogenetic analyses.
The aligned scaffold region and 200 bp upstream from the start of the alignment were extracted.
For reasons of comparability with MIF descriptors, sequence descriptors based on the alignment were calculated.
Segments of the alignment were assigned to structural motifs based on the KcsA structure.
The resolution of the meshes was not relevant, because the images for the alignment were generated in-silico projecting exactly the same models.
Gaps in the alignment were not used in the reconstruction.
Ambiguous regions in the alignment were excluded from phylogenetic analyses.
Gaps within the alignment were treated as missing data.
Gap sites in the alignment were not used in the phylogenetic reconstruction.
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