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The alignment was performed using DNAMAN software.
The alignment was performed using 60 bp window and a cutoff score of 50% identity.
The alignment was performed using BLAT41 ("−fine −maxGap = 3" for maximum sensitivity) using the known plasmid as the reference sequence.
The alignment was performed using default parameters.
The alignment was performed using MAFFT version 5.64 [ 47].
Further manual editing of the alignment was performed using Se-Al [ 82].
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The alignment is performed using Twin (Muggli et al. 2014) (with default parameters) and then these results are filtered according to the χ 2 model mentioned above.
The alignment is performed using pairs of trajectories acquired simultaneously with two labeled proteins: Abp1-mCherry serving as a reference, and a protein of interest tagged with GFP.
Calculation of percentage of insertions/deletions (indels) in the alignments was performed using an in-house tool written in C++.
The alignments were performed using a local version of NCBI BLAST (blast2seq) [ 33] and perl script.
The alignments were performed using the CLUSTALW (1.81) Multiple Sequence Alignments program using default options unless stated otherwise.
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