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The alignment was visually inspected; ambiguously aligned sequences were removed.
The alignment was manually edited to remove poorly aligned residues.
The alignment was performed with software BLAST.
The alignment was weighted using the T-coffee TCS function.
The quality of the alignment was assessed by blending or flickering between the adjacent images.
The alignment was trimmed to ensure it contains only the RdRP sequences.
The alignment was performed using DNAMAN software.
The alignment was again checked under fluoroscopy in both the standard anteroposterior and the lateral planes.
The first sequence from the alignment was confirmed by three sequencing results.
Thus the total number of sequences used in the alignment was 18.
When GO was functionalized with magnetic iron oxide (Fe2O3), the alignment was completed in several seconds.
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