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We have quantified the alignment obtained with this method on the macro, micro, and nanoscale, and it is found to be similar to other alignment techniques such as stretching, slicing, and wet spinning.
We compared collagen fiber alignment obtained by second harmonic generation (SHG) microscopy with the alignment obtained by diffusion tensor imaging (DTI) throughout the wall of a porcine carotid artery to check the feasibility of using DTI as a fast and non-destructive method instead of SHG.
In Figure 23 the Venus dataset is shown for three alignment stages: (a) is the initial image condition for the dataset; (b) shows the alignment obtained after executing our multi-view pipeline; (c) represents the dataset after executing our global optimization method [32].
An improvement in the values for these two parameters for aSVR would reflect an improvement in the alignment obtained using PBs.
Surprisingly, alignment of the positive antigenic peptides with the full HA protein shows a distribution strikingly different from the alignment obtained using the mouse antisera in several aspects.
Our algorithm proceeded as follows on the alignment obtained.
Similar(41)
We estimated that the alignments obtained in Reconstruct starting at a central section with no obvious artifacts required on average ~10 15% overall scaling of the images for the apical and oblique dendrite volumes (Table 1 (available online only)).
A comparison of the project alignments with the alignments obtained according to the proposed method gives good results with small errors relative values, with maximum values of less than 4%.
The alignments obtained from each of the 8 programs were compared to the corresponding reference alignments.
Gene structures were defined based on the alignments obtained, the conserved positions of introns in homologous bZIP genes, and the presence of canonical splicing sites (GT-AG).
For the UCSC sequences, we use the alignments obtained from the UCSC genome browser.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com