The alignment indicates that all four proteins are closely related and likely arose from a common ancestor, although only PPR-B possesses a complete third PPR motif.
The alignment indicates that the β-hairpin preceding the DFG-motif is structurally conserved, even in atypical kinases, and the majority of the DFG-out structures have similar DFG-motif conformation.
The alignment indicates that 40 amino acids are strictly conserved, and structural comparison of C-S lyase with cystalysin indicates similarity in overall fold as well as in active site residues.
However, the alignment indicates that the actual motifs are more complex and that, among the short GDHs, the main differences arise from the nature of the residues in the second and the last position of the motifs.
'*' indicates that the residues or nucleotides in that column are identical in all sequences in the alignment; ':' indicates that conserved substitutions have been observed, according to the physicochemical properties as described for ClustalW; '.' indicates that semiconserved substitutions are observed.
The alignment indicates that the overall amino acid sequences among PriB proteins are not highly conserved; only 21 amino acid residues are significantly conserved: Asn3, Gly9, Ser20, Pro21, Gly23, Glu32, His33, Ser35, Glu39, Arg44, Ser55, Gly56, Gly69, Gly76, Phe77, Val91, Leu92, His93, Ala94, Ile97, and Gly103.
Visual inspection of the alignment indicated that one of three consecutive deoxythymidines (or T s) was deleted.
Visual inspection of the alignment indicated that in the majority of cases, a major peak was surrounded by lower peaks within approximately 60 bp on either side.
Representatives of each of groups (a - e) conta - eer or thr at this point in the alignment, indicontainthat thiseror is a candidathrsite for phosphorylation in othis FA pointins.
The correspondence in function of mapped clusters obtained from the conserved graph of the PINALOG alignment indicates that the resulting alignment shows the actual biological equivalence between the two input networks.
The sequence alignment indicates that the highly repeated 565-bp-region is indeed lost in the Adiantum cp genome.
