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Six predicted maize proteins with more than 55%% sequence identity to rice PSTOL1 were selected and aligned using ClustalX software version 1.83 [ 80]; the alignment included OsPSTOL1 and the Arabidopsis protein kinases SNC4 [ 81] and PRK5 [ 82].
The alignment included endoglucanases from Serratia proteamaculans strain 568 (A8G820, A8G820_SERP5), S. liquefaciens (S5EHX1, S5EHX1_SERLI) and E. coli O157 (Q8X5L9, GUN_ECO57).
The alignment included 64 samples; 49 of Gracilaria salicornia from this study and 15 species of Gracilariaceae from GenBank, with 661 nucleotide positions.
The alignment included worldwide DENV-3 E gene sequences retrieved from the GenBank.
The alignment included 165 sequences and was 217 amino acids long.
The alignment included the same amino acid positions that were used for the phylogeny.
The alignment included none variable sites and none parsimony informative sites.
The alignment (including gaps) is available in Additional file 5: Figure S4.
The alignment includes 18 full length copies and 22 incomplete copies.
In total, 13 positions in the alignment included gaps (Additional file 2: Figure S2).
The alignment includes 365 unique sequences derived from metagenomic sequences (~400 total representatives), in addition to the example in ' Cand.
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