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This measure is completely independent of bootstrap analyses, which only provide information on the support of the different partitions from the alignment in which the tree is based.
A degree of subjectivity regarding these decisions was allowed, depending on the degree of variability of the exact region of the alignment in which they occurred.
This function prints a coloured RC-MSA representation of the sections of the alignment in which the reference alignment and the alignment to be inspected differ.
Columns in the alignment in which gaps had been inserted in regions with insertions, and deletions were stripped before the analyses.
We examined the distribution of SNPs across the genome by recording each site in the alignment in which one of the strains differed from the other two.
Note that the alignment diverges most strongly both in length and in sequence in the loops immediately downstream and upstream of β 3. Figure 6A shows a condensed version of the alignment in which the diverging intervening loops are indicated only by the number of amino acid residues.
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We only saved the alignments in which each species has its corresponding ortholog.
Gapped sites and CpG dinucleotide sites were excluded from the alignment blocks, and the alignments in which 80% or more of sites remained were subjected to the subsequent analyses.
In TASSER [ 20], we assemble the protein models directly using the continuous fragments from the PROSPECTOR_3 alignment, in which the conformations are searched in an "on-and-off" lattice system, i.e. the threading-aligned regions are modeled off-lattice and the unaligned loop regions on-lattice.
An example of the merged alignment in which the hypervariable region was further hand-curated is given in Figure S2d.
These alignment ambiguities are also illustrated by the DIALIGN alignment in which 406 of a total of 910 columns had a quality score of only 0 or 1 and were excluded from further analyses.
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