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However, the alignment from which we inferred the gag tree was manually constructed and its accuracy tested by comparative analyses.
I trust that the authors would agree with me that the statement "the nearest tree neighbor of an eukaryotic mitochondrial protein comes from a taxon other than Rickettsiales" makes little sense when a bona fide ortholog of the eukaryotic protein exists in Rickettsiales but has not been included into the alignment from which the tree was inferred.
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As alluded to above, the equilibrium GC content of the alignments from which the intronic 64×4 matrices were estimated is often much higher than the equilibrium GC that the matrices generate.
For instance, we have observed that the relative 64×4 mutabilities are mostly much higher than 100% when they are estimated using coding regions whose GC matches the actual GC of the alignments from which the substitutions used to estimate mutation matrices were retrieved, rather than the equilibrium GC generated by the estimated matrices.
Consensus sequences were derived from the alignments (from which profiles were constructed) and used to identify their corresponding superfamilies, using default parameters.
The browsing page provides a list of the TAA domains captured by daTAA, each hyperlinked to its own page with an image of the domain structure (where known), a plot of average side-chain size and hydrophobicity, the phylogenetic spectrum and an option to show the multiple alignment from which the HMM was computed.
If the best sequence found in Database B belongs to the multiple alignment, from which we started the matching procedure, then this multiple alignment belongs to the set of BBHs.
After aligning a given sequence (i.e. target) to all the templates in the database, we need to pick up the best alignment, from which we can build a 3D model for the target.
Molecular phylogenies based on sequence alignments are only as accurate as the alignment data from which they are produced; consequently it is important that the alignment data quality is determined [ 33- 35].
Given DNA sequence alignments from multiple species, PhastCons outputs a probability score for each base pair in the alignment indicating from which state the sequence arises.
At each extremity there is a great bend in the system's alignment, from which a number of lower mountain ranges and hills spread out.
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