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Therefore, we have kept the alignment figures as is.
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Alignment in figures as well as phylograms are as shown by the ClustalW2 program web pages, with some modifications.
We find that the probability density distribution of the square of the raw binding-site similarity score fits EVD that is only dependent on the alignment length, as shown in Figure 1.
To glean some insights into the roles of the sites that positive selection and functional divergence might have, we mapped these sites onto the model as well as along the sequence alignment (Figure 3, B, C and D).
The analysis was conducted by providing the structure of Mtb BfrB and multiple sequence alignment (Figure 3) as input to the program.
The Alignment for Figure 2 is presented as Additional file 2. For each Neighbor Joining tree [ 38], the percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) were determined [ 39].
To highlight this, we also marked the residues in the "a" and "d" positions of the coiled-coil heptad repeats on the alignment in Figure 1 figure supplement 1A, as requested.
This transition is represented in the alignment of Figure 2. All these substitutions were silent as shown by alignment of sequence R in BLASTX (NCBI; not shown).
The alignment is available as Figure S1.
Then, an 18 bp footprint sequence covering each detecting regulatory motif was picked out for the alignment as shown in Figure 1(a).
The 30 conformations of the C-terminus of OxB were homology modeled on the basis of the 30 conformations of OxA using the alignment as shown in Figure 2A with MOE (version 2010.10, Chemical Computing Group).
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