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Positive selection was further investigated using a sliding window approach, and adding the mRNA sequences of E. multilocularis to the alignment containing the distinct eg95 clones (Fig. 4b).
For each block, b in the alignment containing nb sequences and mb columns, the ith column of the block is assigned a score Cbi = 1 if all the residues in the column are aligned correctly, otherwise Cbi = 0.
3067 bp of the alignment containing the control region between tRNA-Pro and tRNA-Phe were not well aligned due to high sequence divergence and were therefore removed, resulting in an alignment of 16,352 bp for phylogenetic analyses.
For several other MIRNA arm-gene alignments, such as those containing miR862, miR447a and miR853, the miRNA-region of the alignment is similar to, or weaker than, the alignment containing flanking arm sequences (Figure 5).
Sites from the alignment containing gaps were removed.
All positions of the alignment containing missing data (?s) were removed, resulting in 8,327 aa sites (8,319 informative).
Similar(39)
We retrieved the corresponding metazoan nucleotide sequences from Genbank and aligned them with DIALIGN, specifying that the alignment contained both coding (exon) and non coding regions (introns).
The alignment contains a number of gaps higher than 30% of the aligned bases.
In total the alignment contains 7.5 million SNPs, 6.6 million of which are biallelic.
4,903 positions of the alignment contained gaps in at least one of the taxa.
The alignment contains five protein-coding genes (APOB, RAG1, IRBP, vWF, and BRCA1) for 24 taxa.
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