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Galactose (2% final concentration) was added to the medium after collection of the 0 hours image series.
The urine, after collection, was centrifuged and the supernatant stored at -80°C until all animals' urine had been collected.
No practitioner dropped out of the QILC after collection of baseline data.
Dissections were carried out within the hour after collection.
Blood samples were processed within the hour after collection.
Almost all pupae eclosed on the day after collection.
Egg clusters composed of 25 - 70 zygotes were harvested the morning after collection.
Semen samples were shipped with freezer packs, and analyses were performed on the day after collection.
The fecal samples, on the other hand, after collection were dried out in a drying oven at 40 °C.
Samples were transported to the field laboratory at the Tongliang County Hospital immediately after collection.
The time courses of the FeNO levels after collection are shown in Figure 1.
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