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Aggregation can decrease the affinity of interactions between synthetic dyes and nanotube surfaces [17].
The affinity of interactions between the proteins of the multi-interface hub tended to be higher than that between the proteins of the single-interface hub [4].
Taken together, this suggests that heparan sulfate (or heparin) binding to 67LR may reduce the affinity of interactions between 67LR and laminin, but not abolish them altogether.
Due to the intrinsic variability in the affinity of interactions between a TF and its co-regulators, it is inevitable that the ChIP-seq signal of these types of studies would span a broad spectrum and that the weak signal scenario, as in this study, would be likely to occur often.
Further evidence supporting this dynamic behaviour is the observation that the affinity of interactions between protomers is relatively weak outside of the bilayer environment, as show by the rapid dissociation of protomers upon the addition of a non-ionic detergent (Supplementary Figure 7).
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The assembly of whole cellulosomal subunit depends upon the affinity of interaction between dockerin domains having duplicated sequences and cohesin domains containing repeated sequences, as this interaction provides the most favorable arrangement for the enzymes to act efficiently on the substrate (Jeon et al. 2012).
In many cases, however, it has been difficult to detect these receptor-ligand interactions by conventional techniques, because the affinity of interaction between these membrane proteins is generally low (Kd∼1 µM) [2].
We have previously speculated that AID's ability to potentiate CSR may critically depend on the affinity of interaction between its NES and Crm1 exportin.
This suggests that the affinity of interaction (viz the dissociation constant) between the crystallins & the dementia peptides do not correspond strictly with their pathogenic effects in the organ culture, pointing towards specificity in catractogenesis caused by redADan.
Furthermore, whilst the N-terminal region modestly enhanced the affinity of interaction with GFRP, high affinity interactions were still observed between T-GCH1 and GFRP.
The affinity of the interaction between Norrin and Fz4CRD is significantly different between this study (∼1 micromolar by SPR) and the prior study of Ke et. al. (∼10 nM by Alphascreen and the Octet Red instrument).
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