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The department of one of the authors who co-authored all of the below papers has found that the affi liations were not correct.
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The Affi-Gel matrix has a blue dye named Cibacron Blue F3GA, with natural affinity for native albumin.
Heparin is a linear glycosaminoglycan able to bind to a wide range of proteins with some exceptions, including PvNod41, so it was employed to remove contaminating proteins present in fraction A. The Affi-Gel Heparin Gel flow-through fraction contained PvNod41 that was practically pure (fraction B, Figure 1).
In the radioactive assay, the ProCA1-affi binding with 153Gd3+ was used to treat the cancer cells; the radioactivity of the cell pellets was measured by γ counter after washing 5 times.
Interestingly, while the ProCA1-affi-m was largely concentrated with the CD31 staining in the tumor, the anti-HER2 antibody was not detectable by the immunofluorescence staining analyses.
The ProCA1-affi-m injected into the xenografts was concentrated in ∼5 mM in HEPES buffer, pH 7.0.
The NaTrxhrec-Affi-gel affinity column used was the same as previously reported [ 22].
Western blot analysis of eluted proteins showed that CDK9 was bound to the flavone-Affi-Gel beads but not to the control beads.
The ProCA1-affi and ProCA1-affi-m were incubated with the two kinds of cells at 4 and 37°C, respectively, for 1 hr.
Fluorophore-labeled anti-TNT antibody was incubated with the modified Affi-Gel resin until binding equilibrium was reached.
The ProCA1-affi and ProCA1-affi-m (modified by PEG) were diluted with 10 mM Tris buffer, pH 7.0.
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