Sentence examples for the activity was terminated from inspiring English sources

Different proteolytic enzymes including pepsin, trypsin, chymotrypsin, proteinase K and pronase E were incubated with peptide for 6 h at 37°C and the activity was terminated by heating at 80°C before the peptide was used for assay.

After a 15-min development time, the HRP activity was terminated using dilute sulphuric acid and the relative absorbance at 450 nm was measured using a Spectromax ELISA (Molecular Devices, Sunnyvale, CA, USA) plate reader.

Viability assay different concentration of trypsin (from 0.0002 to 2 mg/ml) incubated with peptides for 1 or 6 h at 37 °C, and the enzymatic activity was terminated by additional heat inactivation at 60 °C for 20 min.

Then, the enzymatic activity was terminated with equal DMEM containing 10% fetal bovine serum (FBS).

After the treatments, laccase activity was terminated by boiling (10 min), and the hydrolytic enzymes Celluclast 1.5L (10 mg g−1 DM) and Novozym 188 (500 nkat g−1 DM) were added, together with NaN3 (0.02% (w V−1) final concentration) to avoid microbial contamination.

After the treatments, laccase activity was terminated by boiling (10 min), and solids were separated by centrifugation and washed three times with pH-adjusted ultrapure water (pH adjusted to 2.5 with HCl).

Enzymatic activity was terminated by the addition of an equal volume (10 μl) of detection reagent and yielded a final concentration of 0.5× stop buffer, 1 nM AMP-Alexa633 tracer, and 2.5 μg/ml ADP antibody.

Incubations were for up to 30 min at 30°C, and PP2A activity was terminated by the addition of 0.1 vol. of 50 μM okadaic acid/2 μM calyculin A. Immunoprecipitates were washed with Buffer C, centrifuged (10000  g for 5 min at 4°C) and the pellets extracted into 50 μl of 2×sample buffer for subsequent immunoblotting.

Dispase activity was terminated by washing the globes three times in DMEM (high glucose and L-glutamine) plus 10% FBS (Gibco/Invitrogen, Carlsbad, CA).

After incubation activity was terminated by washing the cells three times with cold PBS and scraping into 250 μL of PBS.

Dispase II activity was terminated by washing the globes three times in DMEM/F12 (Dulbecco's modified Eagle's medium: Ham's nutrient mixture F-12) medium plus 15% (v/v) FBS.