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Different proteolytic enzymes including pepsin, trypsin, chymotrypsin, proteinase K and pronase E were incubated with peptide for 6 h at 37°C and the activity was terminated by heating at 80°C before the peptide was used for assay.
The sharp decrease of the fluvial activity is terminated by two successive paleosols of Pleistocene Holocene transition.
It is technically almost impossible to evaluate application times shorter than 15 s in such a test because the disinfectant activity is terminated by immediate dilution.
NRF2 activation extends to the late stage, while HSF1 activity is terminated by the end of the intermediate stage.
For a short moment they can no longer transmit any electrical signals; the life-threatening chaotic activity is terminated.
MMP activity is terminated in the brain mostly by four secreted tissue inhibitors of metalloproteinases (TIMPs) (see review by Huntley [6]).
By the action of ACE-2, Ang II activity is terminated by its conversion to Ang 1 7.
Arf1 activity is terminated by its interaction with a GTPase activating protein (ArfGAP), which stimulates the hydrolysis of GTP to GDP.
After a 15-min development time, the HRP activity was terminated using dilute sulphuric acid and the relative absorbance at 450 nm was measured using a Spectromax ELISA (Molecular Devices, Sunnyvale, CA, USA) plate reader.
Viability assay different concentration of trypsin (from 0.0002 to 2 mg/ml) incubated with peptides for 1 or 6 h at 37 °C, and the enzymatic activity was terminated by additional heat inactivation at 60 °C for 20 min.
Then, the enzymatic activity was terminated with equal DMEM containing 10% fetal bovine serum (FBS).
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