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However, the edit paths inferred using EEEP suggest an important role for genetic transfer in the evolution of microbial genomes as well.
HorizStory recovered an edit path from every tree of size 5, 10, and 15, but many of the edit paths in larger trees could not be recovered in less than five hours.
Click the "Edit paths by nodes" button.
To edit and fine tune the result, click on the "Edit paths by nodes" button the the left (or F2) and move the nodes or the curves.
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In Additional file 1: Figure S2c and Sd, the edit path connects two non-overlapping confidence sets.
BP is less reliable than BP*, as it is much more likely that some of the mutations used in the perturbations cancel each other or cancel some of the mutations on the edit path between the two sequences.
In EEEP, the edit path determination is carried out in a breadth-first fashion, where candidate SPR operations are performed on the current modified version(s) of the reference tree with an edit path of length l before any paths of length l + 1 are considered.
As these transfers are analogous to lateral gene transfer (LGT) events, the edit path output by the program corresponds to a minimum set of lateral gene transfers between strains in the reference tree.
Additional file 1: Figure S2a and Sb shows those instances where the trees on the edit path are always within the confidence set of optimal trees of at least one alignment.
Click on the edit path by node icon.
For those trees that manifested conflict, we evaluated the shortest edit paths required in order to reconcile their topologies, using the EEEP algorithm and the AU test (see methods).
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