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For the second round of genotyping we concentrated on those SNPs that had been shown to increase/decrease the GMT of anti-HBs level in the 1st screen by a factor of 1.5 and/or be associated at the level of P<0.005.
These individuals tended to be younger and all were consistently anti-HBc negative, due to the initial selection procedure applied for the 1st screen (see above).
The statistical analysis for the 2nd screen was carried out in an identical manner to that of the 1st screen for anti-HBs level as the outcome.
We studied two outcome measures in the 1st screen (i) peak anti-HBs level as quantitative variable and (ii) anti-HBs status as binary variable, and for the 2nd screen peak anti-HBs level only.
For the 2nd screen all models including the basic model (i.e. excluding genetic factors, see Table 2A) showed an effect of vaccine group and measurement time on anti-HBs level, as expected and as seen in the 1st screen.
There are some limitations to our study: The number of polymorphisms tested in the second sample is small and for technical reasons we could not obtain data for all 'top' SNPs identified through the 1st screen.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com