The Ca2+ indicator fluorescence by ionomycin decreased at approx. 20 min, but that by capsaicin was sustained until at least 60 min.
Intracellular Ca2+-dependent fluorescence arising from the GCaMP6s indicator was monitored simultaneously with action potential measurements using epi-illumination and a photomultiplier tube (D104, PTI).
By doing so, we have shown that the construction of the I2 indicator variable is valid.
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We monitored membrane potential and Ca2+ signals using the ratiometric fluorescent membrane potential indicator di-4-ANEPPS and the Ca2+ indicator rhod-2 in the same preparation.
After hardening, phage preparations were applied in 10 µL aliquots directly on the soft agar with the R36A indicator strain.
Mn2+ quenching of the Ca2+ indicator dye revealed substantial sustained divalent cation influx into the hyperpolarized cone IS.
Intracellular Ca2+ in RAW 264.7 cells was measured fluorometrically using the Ca2+ indicator Fura-2.
We measured [Ca2+] i levels using the Ca2+ indicator fura-4.
