The duplicate freezing and de-thawing of all samples represent potential sources of error, though the equal treatment of all samples should minimize the influence upon the differences found between laboratories.
ApoA1, ApoB, and CRP were determined with immunothurbidimetric methods (all from Roche Diagnostics Scandinavia AB, Bromma, Sweden) in thawed plasma/serum of all study individuals at the accredited local clinical chemistry laboratory.
The median cell viability after thawing of PBMC from all six donors was 95%% and decreased only slightly after resting (93.5 %), indicating a high quality of utilized cells.
The outcome of all thawing cycles up to November 2009 have been analysed, including the cycles in which embryos were thawed for transfer and also those in which the couple did not wish to have embryos transferred, but consented to have them thawed and their morphology assessed before they were discarded.
To assess the impact of survival of cryopreservation and thawing with all blastomeres intact on the outcome of frozen embryo replacement (FER) cycles.
The main deteriorations of asphalt pavements in cold regions are due to the effect of heavy traffics, water action, low-temperature fluctuations, freeze-thaw cycles and the combination of all these factors together.
Before infection, aliquots of all strains were thawed for 1 h at 25°C.
Cryopreservation of tissues depends on the presence of cryoprotectants, their addition and removal, and exposure to conditions during freezing and thawing, all of which depend on cellular membrane permeabilities to water and cryoprotectant.
To avoid repeat freezing and thawing, all of the plasma transported to IBT was divided into 1.5-mL tubes and stored at −70 °C.
The viability of cells immediately after thawing across all participants is shown in Fig. 2a.
