Sentence examples for thawing media from inspiring English sources

To investigate the effects of brain-derived neurotrophic factor (BDNF) supplementation to freezing and thawing media on frozen-thawed human sperm parameters.

The ovary and pedicle were placed in a perfusion tray and immersed in and perfused with warm thawing media.

Donated cleavage-stage frozen embryos were thawed using standard commercial kit (COOK thawing media) and then cultured to blastocyst stage using Blastocyst medium (COOK, Australia).

Three thawing media were used, consisting of Leibovitz L-15 media supplemented with 10% FCS and reducing concentrations of DMSO (1 M, 0.5 M, 0 M).

Sucrose was included only in the first thawing media at 0.1 M. All three thawing media (pH 7.3 7.4) were filter sterilized prior to use and perfused warm (37°C) through the ovary for 10 min at a rate of 1 ml min−1.

Early methods using the former technique involve the use of glucose to off-set the osmotic changes during DMSO removal but supplementation of thawing media with glucose has also been shown to significantly decrease caspase-3 activation and increase the preservation of adhesion molecules, suggesting that this basic addition may be advantageous in more than one way (Tolosa et al. 2011a).

When I visited Havana last year, it seemed as if a culture and media thaw was under way.

For the large-scale screen, for each batch of 30 plates carrying the genome-wide siRNA library, 2 vials of cells were thawed in MEF media.

For cytokine secretion assays, PBMC were thawed in warm media and adjusted to a final concentration of 2 × 10/ml in RPMI-1640 medium supplemented with 10% v/v heat-inactivated fetal calf serum, 2 mM glutamine, penicillin/streptomycin and 20 mM HEPES.

Seeds were sterilised after thawing, before sowing onto media as described above.

It will be possible to use these data to improve the modelling of coupled thermo-hydro-mechanical phenomena involved during the freezing and thawing process in porous media.