The frostiness did eventually thaw with a sweet, though unseasonably sunny, ending, which hinted at a below-stairs pairing in bloom.
Relations subsequently thawed, with Cameron vowing to build a "lasting friendship" with Beijing during a December 2013 visit to China and Chinese premier Li Keqiang meeting the Queen during a visit to Britain the following year.
To control for inter-experimental variation in CD3 zeta-chain mean fluorescence, cells from a single donation, from a healthy donor, were cryopreserved in aliquots and one aliquot was thawed with every experiment to establish a normalization factor.
They are thawed with no decrease in activity.
On a recent rainy night we thawed out with a pale-orange, creamy shrimp and lobster chowder loaded with seafood.
One fourth (25%) of each harvested sample (see Cell transduction) was thawed, pulverized with a pestle, and then loaded into microplates along with conditioned media samples and standards.
Tissue samples were thawed, homogenised with a Teflon pestle and run under reducing and denaturing conditions on 10% SDS PAGE.
Aliquots of plasma were thawed, spiked with a naproxen internal standard, and the serum proteins were precipitated as described previously (Teng et al. 2003).
Cell culture supernatants were harvested and stored in microtubes at −80°C, and TNF- α, IL-10 assays were performed on freshly thawed samples, with a standard quantifiable sandwich enzyme immunoassay technique.
Then towards the end of the year, relations with the United States began to thaw, with Sudan being removed from a watchlist of countries deemed to be facilitating the financing of terrorism.
