Sentence examples for thaw method from inspiring English sources
Genomic DNA of the bacterial symbionts was isolated by the freeze and thaw method (Radjasa et al. 2007b). 1 ml suspension of bacterial cell was centrifuged at 14,000 rpm for 10 min, and supernatant was then discarded.
Plasmids purified from E. coli cultures were transformed into A. tumefaciens GV3101 using the freeze thaw method [ 33].
The construct harboring 35S- SaSce9, was introduced into Agrobacterium by freeze thaw method and transferred into wild type Columbia ecotype of Arabidopsis by floral dip method [ 59].
Gene constructions were transformed into Agrobacterium strain GV3101 by the freeze thaw method, and the transformants were grown overnight in Luria-Bertani (LB) medium.
Each recombinant construct was separately transformed into Agrobacterium tumefaciens strain LBA 4404 using a freeze and thaw method [ 40] and transformed colonies confirmed by PCR amplification of 800 and 987 bp bands for CaMV35S (using primers 35S_F and 35S_R) and yoeB Spn -GFP (using primers yoeB_ F and GFP_R), respectively.
The recombinant plasmids pCAMBIA1302-LhSorTGA2 and pCAMBIA1302-LhSorNPR1 weacheach introduced into Agrobacterium tumefaciens (strain GV3101) competent cells via the freeze-thaw method.
A novel hexagonal boron nitride nanosheets (h-BN -doped poly (vinyl alcoh-BN -dopedic acid (PVA-h-BN -dopedpolymer electrolyte (GPE) is first fabricated through a facile freeze-thaw method.
The hydrogels were synthesized using a freeze-thaw method with a modified GO network, which was crosslinked by β-cyclodextrin aldehyde (β-CD-DA) to form an inorganic GO network (β-GO).
Large unilamellar vesicles (LUV) were prepared with PC/cholesterol (8 1 w/w) and PC/PG (7 3 w/w) using the freeze-thaw method as described previously (Mayer et al., 1985; Zhang et al., 1999) followed by extrusion through 0.1 μm double-stacked nuclepore filters using an Mini-Extruder (Avanti Polar Lipids, Inc).
Polysaccharide was deproteinized by repeated freeze-thaw method.
The plasmid was transformed to Agrobacterium strain Agl-1 via the freeze-thaw method.
