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We used protein sequences that were previously characterised or had an assigned function in other species.
We have investigated the effects of FGF-1 and FGF-2 on the behaviour and adhesion properties of human pancreatic adenocarcinoma cell lines (BxPc3, T3M4 and HPAF) that were previously characterised for the expression of FGFRs.
In PRIME, a prespecified mutational analysis was conducted on banked tumour samples that were previously characterised as KRAS exon 2 WT to test for mutations in NRAS exon 2 (codons 12 and 13), and KRAS and NRAS exons 3 (codon 61) and 4 (codons 117 and 146).
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Because many reference strains were previously characterised using non-genetic methods, it is possible that they were misidentified and, thus, the reference strains themselves may be unreliable [ 32].
The materials were previously characterised in the laboratory.
Ezh1 and EED antibodies were previously characterised ([ 7], [ 23]).
The DPR antibodies were obtained from Dr. Leonard Petrucelli and colleagues and were previously characterised [ 13].
A quantitative TaqMan real-time PCR assay was performed using a standard curve that was generated with a plasmid clone from a Brazilian swine strain that had been previously characterised as having genotype 3 [ 26].
A recent publication by Ashton et al. (Ashton et al., 2012) capitalised on an established mouse model of chronic myeloid leukaemia blast crisis (CML-BC) that arises from the interaction between two different fusion genes (BCR-ABL and NUP98-HOXA9) and results in a functional hierarchy that has been previously characterised (Neering et al., 2007).
Among the five identified HESX1 interactors, DNMT1, SAFB1 and SRFBP1 are nuclear proteins that have been previously characterised [20 22,28,29].
This protein library included all of the E2s that have been previously characterised [ 8, 13, 27, 29].
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CEO of Professional Science Editing for Scientists @ prosciediting.com