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Employing a double-blind, placebo-controlled design, this study examined the acute actions of nicotine (6 mg) in 24 non-smokers performing a visual search task of spatial attention that was probed with behavioral performance measures and the N2pc component of the event-related potentials (ERPs), which served as a neural index of spatial attentional selection.
The sample was subjected to SDS-PAGE and transferred to a membrane that was probed with specific antibody.
Protein (20 µg) was loaded in a 10% SDS page, and electrically transferred onto a PVDF membrane that was probed with the antibodies listed in Table 2. Detection was performed with a standard ECL based system.
Following paired-end sequencing, the MAPseeker software is used to align the reads to the sequence of the RNA that was probed.
Equal amounts of protein were separated by SDS-PAGE (4 12% gradient gels), and proteins were transferred to a polyvinylidene difluoride membrane (Invitrogen), that was probed with primary antibodies.
Next, we analyzed the distribution of errors around the target orientation (orientation of the item that was probed; see Fig. 1c) and the nontarget orientations (those of items in the sequence that were not probed; Fig. 1d).
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As the New York Times reported this weekend, DeVos appears to have effectively dismantled the team of investigators that was probing shady for-profit colleges accused of preying on desperate students.
Blots that were probed multiple times were stripped according to kit protocol.
Table 3 summarizes the results of the PCRs for all segments from all isolates that were probed.
Likewise, immunoblots that were probed with the CbbM-specific antibody provided no evidence that Form II RubisCO was compartmentalized in carboxysomes (data not shown).
In experimental studies that quantified base pair opening using imino-proton exchange, it was the relative probabilities of the stacked and opened states that were probed [15], [16].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com