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As previous studies showed that the resolving power of multi-locus barcodes is almost always higher than that of single-locus barcodes [3], [4], [60], [61], all testing was based on the combined dataset of rbcL and trnL-F.
The straight-only configuration shows that the resolving power increases from 54 to 57 as the electric field is increased from 14 to 20 V/cm.
Pi-affinity SDS/PAGE was conducted at 75 V for 5 h, with the modification that the resolving gel (10% acrylamide) contained 75 μM Phos-tag™ acrylamide (www.phos-tag.com) and 150 μM MnCl2 [ 30].
It is clear that the resolving power curves are linear when plotted in logarithmic scale and therefore the data quality can be summarized by the slope, steeper slopes being better.
The RF amplitude was increased to 320 Vp-p to ascertain whether RF fields would begin to slow ions, but Figure 4A illustrates that the resolving power did not change with the current SLIM configuration, which is important for operating SLIM for ion mobility measurements.
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Before that, the resolved T-F units are merged into segments firstly.
A similar assessment is done for the parameters that describe the resolved resonance range.
The reason for this is related to the fact that the resolved structure is relatively detailed within the upper crust.
The fact that the resolved components in the deconvolute exhibit nearly equal widths suggests that the lifetime is the same for all components.
We note that the resolved Kns obtained from this initial analysis is greater than published values (Kns ∼ 6.6 × 10 M–10.
After that, the game resolved itself into a contest between Rooney and Given.
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