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Some methods compare divergent, aligned proteins to spot discriminating residues that suggest functional signatures in sequences (EFICAz2) [20] or in structures (FLORA) [23].
Our measurements of gene expression under well-characterized conditions directly relevant to defined biological processes illustrate examples of altered gene regulation that suggest functional differences between species.
Finally, gene-gene interactions that suggest functional relationships between pairs of genes are being revealed by large-scale assays for genetic interactions [ 12, 13].
These genes are not only enriched in regulatory and essential genes, but their secondary isoforms possess additional sequence features that suggest functional roles for them distinct from the primary isoform.
To this end, an orthogonal approach relies on 3D templates: small structural motifs built from key amino acid functional determinants that suggest functional similarity when matched geometrically in unannotated proteins (Wallace et al., 1997).
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Microscopic examination of wings infected by G. destructans reveals a degree of damage that suggests functional impairment.
This finding is in line with previous studies that suggested functional problems in color perception problems, particularly in blue in children with ADHD [ 18– 218.
Even more fascinating is the fact that two of these pirated genes, v-cyclin and v-CFLAR/v-FLIP, are co-expressed from the same spliced latent transcript, an arrangement that suggests functional interdependence (Fig. 1A).
Taken together, there is no evidence for compensatory increases in homolog levels that would suggest functional redundancy, despite co-expression of all three in neurons.
These assay systems provide strong corroborating data that suggest a functional significance for this intracellular interaction domain in cell-cell adhesion.
18, 19 There are also experimental models that suggest long-term functional deficits after multiple concussions.
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