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It turns out that subsequent analysis of mutations from different α values also leads to a relatively small difference.
A protein stain should bind irrespective of the protein's composition, relative hydrophobicity or ability to bind SDS, and work reproducibly over a broad range of protein concentrations without interfering with them so that subsequent analysis such as Edman sequencing or MS identification is not compromised [ 81].
This is the first time that field population of Brandt's voles have been typed at the MHC region, we have made every effort to initially ensure that PCR primers do actually amplify single expressed products to clarify that subsequent analysis based on genomic DNA is appropriate [ 80].
The ATP concentration of cell lysates was adjusted to a final concentration of 2.5 mM to assure dissociation of HSP72 from RNP particles so that subsequent analysis of binding reactions between RNP and HSP72 could be performed without interference from pre-formed HSP72-RNP complexes (23).
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The eT project gathered a complex dataset of in vivo and ex vivo data from brain tumour patients, in which QC checks at many levels were performed to ensure that subsequent statistical analysis was reliable.
When comparing protein profiles of endometrial fluid collected using these two sampling techniques (66), both proved to be satisfactory sampling methods that enabled subsequent analysis of uterine fluid components.
Three members of the research team independently read and coded the first three interview transcripts, then met to compare their analyses and developed a codebook that guided subsequent analysis of remaining transcripts.
22 23 We used focus groups to generate the group interaction and raw accounts that would undergo subsequent analysis and interpretation.
We were concerned that this short-term ketogenic feeding paradigm might trigger secondary metabolic changes such as hyperglycemia or dyslipidemia that could confound subsequent analysis.
The text files were batch-imported into OriginPro 9.1 software (OriginLab, Northampton, MA, USA) using a custom LabTalk script that performed all subsequent analysis.
Given that the overlap is relatively small (<10%) and that the subsequent analysis uses a different analytic approach (genotype data from individual subjects), we do not think the overlap is a significant concern.
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