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However, the nodal support based on the combined nuclear dataset was remarkably higher than that retrieved with the combined plastid matrix (Additional file 3).
The evaluation of positive selection using the model implemented in Single Likelihood Ancestor Counting (SLAC) showed three sites under selection, one of those sites being similar to that retrieved with model M8 in PAML.
This result was confirmed by two-step iterative PSI-BLAST search that retrieved with significant E-values a number of methyltransferase domains classified in SCOP (e.g. d1p91a_with E=1e-34, d1kpga_with E=1e-31, d1sqga2 with E=4e-14; sunid=53335amily sunid=53335).
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The several cases of pseudogenes that retrieved hits with small e values – indicating full sequence hits – most likely represent novel pseudogenes that represent more recent gene duplications events and not falsely annotated genes.
Another clue in favor of the hypothesis of a viral origin of RPHM21 comes from the analysis performed with HMMER that retrieved a significant hit with a mitogen (fibroblast growth factor, FGF; i.e., a factor that triggers mitosis) of a baculovirus.
A small proportion of two hits revealed sequences that retrieved significant matches only with animal proteins, but which belong to protein families with members among plants and fungi.
We hypothesize that SSEA3 expression is either identifying another less differentiated and/or tissue specific stem cell like population that is retrieved with the donor skin biopsy or that with culture, a subpopulation of fibroblasts may acquire the ability to express SSEA3.
Supplementary Figure S3 shows the percentage of articles that were retrieved with different search strategies.
The finding of a decrease in GF with the "age" of murine tumours shows that information retrieved with the proposed method may be biologically relevant.
Redundant pharmacophores are pharmacophores that could be removed without decreasing the recall of the set of combined pharmacophores i.e. pharmacophores that only retrieved ligands that were also retrieved with other pharmacophores of the set.
Note that using these mapping methods (or any other API method that retrieves src_compound_ids) in combination with the API methods for retrieving source information (above) allows users to convert retrieved src_compound_ids into URLs programmatically, if required.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com