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Loss of FA resistance mediated by the four mutant FusB proteins could have resulted from the replacement of residues critical for the interaction with EF-G or by prompting gross changes in the protein that prevented correct folding; the latter seems unlikely since all four mutant proteins could be overexpressed in E. coli and purified in a soluble form.
Sequences that prevented correct assembly such as sequences from chimeric DNA were removed and the remaining contigs were re-aligned.
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We develop a technique to correct the distortion generated by the presence of a grating that prevents correct imaging of a test object in an optical system.
The nurses in charge of the patients did not report any relevant adverse event related to the use of the cleaning CSS, neither reported any major issue that prevented the correct use of the device.
The supernatant was treated with 0.25 0.6 U/µl of DNase I (from bovine pancreas; Sigma-Aldrich) and 0.004 0.009 U/µl of RNase A (from bovine pancreas; Sigma-Aldrich) in a 50 mM MgCl2 solution, followed by 30 min incubation on ice, to remove nucleic acids that prevented a correct running during electrophoresis.
As neither C-terminally truncated wild-type receptor localized to the cell surface, we concluded that the truncation also prevented correct trafficking of S1p2 receptors.
Pseudogenes had one or more mutations that would prevent correct translation; each of the inactivating mutations was subsequently checked against the original sequencing data.
Pseudogenes had one or more mutations that would prevent correct translation and each of the inactivating mutations were subsequently checked against the original sequencing data.
Adaptor sequences that would prevent correct assembly of the raw sequencing reads were removed following a similar strategy to that used in NCBI's VecScreen tool, based on the known adaptor sequences used in library preparation.
If a gene contains mutations that could prevent correct expression (i.e. missing start codon or stop codon, non canonical splicing site, frameshift or in frame stop codon), it was considered as a pseudogene.
Some barriers that can prevent correct implementation are unfamiliarity and embarrassment, hierarchy, timing of the checks, duplications with existing processes, lack of communication and modification of the checklist [ 10].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com