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The dimerization interfaces feature unique interactions that prevent heterodimerization.
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Several groups have introduced sequence changes in the FokI dimer interface that prevent homodimerization while allowing the necessary heterodimerization (Miller et al. 2007; Szczepek et al. 2007; Doyon et al. 2011; Ramalingam et al. 2011).
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Moreover, structural differences may prevent heterodimerization of some protein pairs.
To prevent heterodimerization when multiple Tet systems are expressed in the same cell, single-chain variants of the transactivators have been constructed.
In such systems, the Tn 10-derived TetR moiety of tTS was replaced with other natural TetR variants to prevent heterodimerization with rtTA [ 8- 11].
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Pertuzumab is an IgG1 monoclonal antibody that binds to HER2 at the dimerization domain, sterically preventing heterodimerization [ 68, 69].
It has been shown that COUP-TFII has 2 main roles in the RSP, (i) repressing the RSP by directly sequestering retinoid X receptors, thereby preventing heterodimerization to retinoid acid receptors and inhibiting gene transcription, and (ii) modulating the transcriptional activity of GATA proteins.
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