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Serial intravenous or intraperitoneal injections of PAb inhibited tumour growth and prolonged survival in mice bearing murine plasmacytoma, while TUNEL assay demonstrated that PAb induced statistically significant apoptosis (P < 0.05) compared to control treatments.
In addition, the apparent viscosity measurement indicated that PAB had an obvious hydrophobic association property.
Model results show that PAB is effective for the remediation of a PCE-contaminated aquifer, since the concentration of PCE flowing out of the barrier is everywhere always lower than the concentration limit provided for in the Italian regulations on groundwater quality.
This assumes that Pab is the same in both locations.
These results demonstrate that PAb polyclonal antibodies specifically targeted myeloma cells.
These results underscore the specificity of this technique and indicate that PAb will not induce immunosuppression.
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This suggests that PAB-1 is likely to regulate mRNAs that function at different stages of germline development [ 54].
In addition, agarose gel electrophoresis demonstrated that PAb-treated cells exhibited a ladder-like pattern of DNA fragments consisting of multiples of approximately 180 200 bp, consistent with endonuclease-induced DNA fragmentation (Fig. 3d).
Considering that Pab-NTD, which is a structural homolog of DTD, also interacts with the substrate mostly through main chain atoms, it appears to be a conserved feature of this fold to employ main chain atoms extensively for ligand binding (Hussain et al., 2006, 2010).
In the double transgenic animals expressing WT or mutant FUS and PAB-1, under the same basal conditions, the distribution and morphology of FUS were identical to those in single transgenic FUS animals, indicating that PAB-1 does not affect localization of either WT-FUS or mutant FUS animals per se.
To further confirm that these PAB clones bind to human PSP94 protein, we used another ELISA sandwich layout in which in vivo biotinylated PAB clones were immobilized to a coated streptavidin and binding of His-PSP94 was detected by anti-PSP94 antibody followed by a secondary IgG-HRP conjugate (results not shown).
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