Thermodynamic analysis of numerous GA and MBP variants suggests that the key to our approach involved stabilizing the modified MBP and OspA subdomains via external interactions with neighboring substructures, indicating that subdomain interactions can stabilize alternative folds over a broad range of sequence variation.
However, two-state folding not only precludes a detailed view of different stages in folding pathways, but also masks the underlying complexity of a process that involves finding a single structure, stabilized by a myriad of specific interatomic interactions, from the astronomical number of possible alternatives available.
We propose that MT binding reduces the conformational freedom of loop11, stabilizing a helical turn that involves Kin3 and255 (Kin1 VAla260 and Ala260 (Kin3 above4), and Kin3 Thr258 (Kin1 Thr242) above α-tubulin's H3ʹ.
The structure of the core is stabilized by an intron-specific set of long-range interactions that involves peripheral elements.
As the system stabilizes, it becomes more plausible that a single big bank will fail or be rescued in a way that involves large losses for creditors.
Just getting the whales to Key Largo was a multiday process that involved waiting for them to stabilize and then transporting them on a boat equipped with a three-sided berth.
Additionally, processes that involve multi-protein complexes are represented by a single member – one CMG (Cdc45·Mcm2-7·GINS Cdc45·Mcm2-7·GINSes DNA polymerase at eacomplexication fork.
Levels of transient secondary structure for example, may increase (i.e., stabilizing) or decrease (i.e., destabilizing), either enhancing or reducing contributions to interactions that involve conformational selection.
That involves new technology.
For several years our research team has attempted to develop a sampling strategy that involved the use of additives to stabilize thiosulfate in hot-spring waters[23] and suifide-rich ground waters.[60] For example, we experimented with adding zinc or cadmium chloride to water sample to quantitatively remove dissolved sulfide and preserve polythionates and thiosulfate.
