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Wave propagation was visualized by pre-incubating the monolayer with a membrane permeable dye that fluoresced upon binding calcium (Fluo-4).
We observed colorectal superficial areas that fluoresced upon light excitation.
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The amount of unbound siRNA in solutions of dendrimer/siRNA was determined by measuring the fluorescence of a commercially available dye, PicoGreen, that fluoresces upon binding to double-stranded DNA or RNA.
Intracellular ROS generation was verified by staining with DCFH-DA, a commonly used membrane permeable dye that fluoresces upon its oxidation to DCF by intracellular ROS [30].
Generation of ROS was quantitated using DCFDA, a compound that fluoresces upon oxidation by ROS.
Cells were counted using a DNA intercalating dye that fluoresces upon incorporation (CyQuant dye, Invitrogen).
The latter was estimated from the intracellular increased emission of dichlorodihydrofluorescein dye, a chemical that fluoresces upon reaction with oxidative species [ 25].
Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization.
The accumulation of ROS in senescent cells was confirmed by a flow cytometry analysis using H2-DCFDA, a cell permeant fluorigenic probe that fluoresces upon oxidation (Figure S1).
In order to determine the respective CMCs of NEFAs in the buffer (10 mM Tris-HCl, 50 mM NaCl, pH 8.0) used in the study, we used N-phenyl-1-naphthylamine (NPN) as a probe that fluoresces upon binding to micelles, as reported earlier [40], [45].
Mitochondrial superoxide levels were measured by staining MM cells cultured for 60 h without or with DCA with MitoSOX (5 μℳ, Life Technologies), which is a mitochondrial targeted probe that fluoresces upon binding to free superoxide.
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