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Towards this goal, research efforts focused on salivary gland proteins expressed in ticks that fed for 3 4 days or longer, primarily due to the ease of obtaining protein and RNA from fed, compared with unfed, ticks [10], [11], [12].
Specifically, Na et al. noted that flies that fed for just 3 weeks on an HS diet had increased cardiac arrhythmia and that HS-fed flies overall had a decreased lifespan (Na et al., 2013).
Caterpillars that fed for two weeks on plants silenced in the expression of a putative lipid phosphate phosphatase (LLP) and a protein of unknown function (UnkA) gained ~2-fold and ~1.3-fold more mass, respectively, than larvae grown on EV control plants.
In order to obtain enough culture of aphid that fed for several generations on Bt corn plants, a number of 20 30 individuals of R. maidis were released on Bt corn which contained a synthetic Cry2Ab/1Ac gene encoding a nearly full length Cry2Ab protein.
We created cDNA microarrays for Manduca sexta and Heliothis virescens separately, by spotting normalized midgut-specific cDNA libraries created from larvae that fed for 24 hours on MeJA-elicited wild-type (WT) Nicotiana attenuata plants.
These microarrays were hybridized with labeled probes from neonates that fed for 24 hours on WT and isogenic plants progressively silenced in JA-mediated defenses (N: nicotine; N/PI: N and trypsin protease inhibitors; JA: all JA-mediated defenses).
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That fed into our music.
They were the Horn & Hardart Automats, the cavernous cafeterias famous for coin-opened, glass-and-chrome food compartments and for the chicken potpies, baked beans and other low-priced offerings that fed patrons for decades, but now nourish only nostalgia.
Chromatin samples were prepared from the intestines from animals that were fed for 22 days (fed ), fasted for 22 days (fasted ), and fasted for 21 days and then refed for 1 day (refed ).
All PCRs performed on the DNA of nymphal and adult ticks that fed on guinea pigs for different periods resulted in amplicons compatible with R. rickettsii, indicating that all 90 guinea pigs in this study were exposed to R. rickettsii infected ticks.
Ms. Foster, 31, sees the land and the sky as it used to be, feels on her hands the dirt that has kept her family fed for more than a century, and hears the spring peepers that serenade along the fringes of Sagg Pond, just a few hundred yards west of her home.
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