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Fold repression was calculated as normalized luciferase activity expressed from pREm-fLuc divided by that expressed from p19RE-fLuc.
MiR-19 repression of the 19RE reporter expression was defined as the luciferase activity expressed from the REm reporter divided by that expressed from the 19RE reporter.
The response of a reporter to let-7a silencing was indicated by fold repression, which was calculated as the luciferase activity expressed from the REm reporter divided by that expressed from the LRE reporter.
For this, synthetic peptides of 15 aa with an overlap of 11 aa comprising the C1 (pool 1), C2 (pool 4), and V3 (pool 5) regions based on the CRF12_BF sequence (identical to that expressed from the BF vectors) were employed (see Materials and Methods).
The transgenic complementation experiments reported here expressed Lhr orthologs in hybrids in addition to that expressed from the endogenous mel-Lhr gene.
This analysis will detect the transcripts from both the endogenous cyclin C gene and that expressed from the plasmid.
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We have genetically engineered a panel of recombinant measles viruses (rMVs) that express from various positions within the MV genome either the HN or F surface glycoproteins of mumps virus (MuV) or the env, gag or pol proteins from simian immunodeficiency virus (SIV).
We constructed a plasmid that expressed dcmA from the native DM4 promoter as well as clcA from a constitutive promoter.
Messages range from the angry – "Fuck Daesh [Isis]" and "Fuck terror" – to those that expressed solidarity – "From Spain with Love".
We generated mice that expressed Cre from the lymphatic vessel endothelial hyaluronan receptor 1 (Lyve-1) locus and that showed efficient recombination of loxP-flanked genes in lymphatic endothelium.
The 99% School advertised the march in advance, disseminating an email that expressed support from school parents.
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