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PGD2 turnover in intact cells was determined similarly to that described previously [28].
Compression injury was carried out at thoracic level 9 (T9) similarly to that described previously [24], [26], [35], [36].
Ceramide was analyzed using a similar approach to that described previously [44] using N-heptanoyl-sphingosine as an internal standard.
The procedure of the test was according to that described previously [ 16].
This was carried out similarly to that described previously (16, 19, 27).
HBV ribozyme activity assay was conducted similarly to that described previously.
The duration of obesity followed a similar definition to that described previously.
Recombineering was carried out using a slightly modified protocol to that described previously [ 10].
The concentration of lovastatin in the FRS extract was quantified using HPLC according to the method that described previously [ 24].
NK and T cell degranulation assays were performed in a similar manner to that described previously (Prod'homme et al., 2010).
The 'atypical index' was calculated using a method similar to that described previously (Robertson et al, 1994).
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