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Identification of polymers that can template nanoparticle synthesis under ambient conditions, without the necessity of additional reducing agents, derivatization chemistries, or harsh synthesis conditions, offers significant advantages over other existing methods, particularly over those that utilize pEI25k.
For microtubules, the best characterized nucleator is the γ-tubulin ring complex, which has 13 copies of the protein γ-tubulin (a paralog of α- and β-tubulin) and then some other proteins that hold them in a slightly distorted ring that can template the growth of a microtubule with 13 protofilaments [ 38, 39].
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Both the overall length of the RT-PCR products and the extensive region of similar sequence identity in the middle that can promote template switching (see below) confound RT-PCR analysis of the six possible EDA and V exon combinations.
At the end of the first phase, EXMOTIF has computed the pos-lists for all simple motif components that can satisfy the template.
In addition to DNA templates, we also attempted to identify polymerases that can use GNA templates.
E scherichia coli and Salmonella contain numerous classes of repeat elements that can serve as templates for exchange (Rocha 2004).
The key for this PCR screening is a critical annealing temperature (Tc) that can discriminate mismatched templates from the matched desired mutant.
This approach applies x-ray crystal and NMR structures to identify pockets within the protein that can serve as templates for which compounds can be designed to potentially interact.
As before, we propose the possibility that Arabidopsis plants harbor a cryptic store of sequence templates that can overwrite the parentally contributed genomes by a template-directed mechanism.
The German merger model is not a template that can be applied on a European scale.
The lessons have created a template that can be used by other NHS Trusts to copy the initiative.
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