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Nine rounds of FACS were used to isolate mutants that bound with high affinity to αvβ3 integrin (Table S2).
Among the glycopeptides that bound with medium affinity to Aq (20 55% inhibition), two subgroups could be distinguished with respect to their T-cell responses.
The strategy proved successful when applied to the glycopeptide CII259 273 from type II collagen and led to novel glycopeptides that bound with variable affinity to the class II MHC Aq and DR4 receptor proteins.
We recently used yeast surface display, a combinatorial method, to identify EETI-II-based knottin peptides that bound with high (low nM) affinity to αvβ3/αvβ5 or αvβ3/αvβ5/α5β1 integrins [8], which are overexpressed on tumors or their neovasculature and mediate angiogenesis and metastasis [18] [21].
A common feature of the glycopeptides that bound with medium affinity to Aq, but induced weak or non-existent T-cell responses, was the presence of a large side chain in p263, i.e. cyclohexylalanine in 6 and 9 and m-methylphenylalanine in 8, 11, and 15.
To tune the strength of the translational inhibition, we replaced the L7Ae protein, the K-turn RNA motif, or both with variants that bound with weaker affinity.
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M protein and M-like proteins are major virulence factors that bind with high affinity to the Fc-part of IgG.
CRT searches for genes in the susceptibility genetic loci that bind with common transcription factors.
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CEO of Professional Science Editing for Scientists @ prosciediting.com