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At the second recombination event, a plasmid-borne sequence is coupled to a chromosomal sequence, forming a new allele that is selectable.
The truncation of some integrated T-DNAs at their left end along with the occurrence of independent segregation of multiple T-DNAs unintendedly resulted in a single-copy segregant that is selectable marker-free and homozygous for the gfp gene.
Despite the targeted nature of IDH inhibitors, IDH mutation likely unleashes epigenetic marks that are themselves selectable, such as DNA methylation.
At least one of the selected tracks must be a Hi-C (3D) track, and the accompanying selected tracks (called 'query tracks') determine the types of statistical analyses that are possible, and therefore selectable in the system.
Strains that lose the URA3 insertion result in derivatives that are Ura- and selectable on medium containing 5-fluoro-orotate (Boeke et al. 1987).
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Direct integration in WT haploid strains (BY4741) and selection for the URA3 marker results in yeast colonies that are red following establishment of the selectable marker.
Hirst has discussed the various aspects of structural soundness (e.g., folding stability or speed) that are singled out as proxies for selectable function [9].
Therefore, transgenic sweet orange and citrange lines carrying the selectable marker genes that are most commonly used in citrus transformation are substantially equivalent to the non-transformed controls during long-term agricultural cultivation.
