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The nutrient assimilation and the DHA and TFA production were measured, the productivity and yield [% chemical oxygen demand (COD)] of the DHA and TFAs were calculated, and the fatty acid (FA) composition was analyzed.
The trend of DHA and TFA productivity (Table 2) in the mixotrophy is similar to that of DHA and TFA production.
The TFA production and composition are shown in Figs. 3, 4 and Table 3.
Rasdi and Qin (2015) found the N P ratio influenced the DHA and TFA production from T. lutea.
The TFA production of the mixotrophy was not obviously higher than the autotrophy (p > 0.05) at day 8.
Therefore, T. lutea might not be an optimal microalgal strain for DHA and TFA production from organic carbon.
Similar(44)
Therefore DHA and TFA productions of mixotrophic culture were higher than those of autotrophic culture and heterotrophic culture, due to the higher content of DHA and TFA and biomass concentration in the mixotrophy.
In order to assess the potential for lipid production of each strain, TFA productivity should be compared, since the TFA productivity represents the capacity of the strain to convert received light, which was the same for each culture, into the desired compound per unit of time.
The two major dietary sources for TFA include production via industrial hydrogenation of vegetable oils (partially hydrogenated vegetable oils, PHVO) and through bacterial hydrogenation in the rumen.
Although the mixotrophy enhanced the DHA content and production, the DHA production directly from the glycerol was not very high (16.19 mg/L) and the DHA yield (2.6% COD) and the TFA yield (13.1% COD) were also very low.
Since TFA-adduct production is induced with a single dose of halothane and persists in measurable concentrations in rat heart for greater than 90 hours (but less than 10 days), [ 8, 33] the possibility for cross-reaction with MAA-adducts is plausible.
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