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For all tests worms, 20 animals/plate by triplicates were scored.
For the paralysis tests, worms grown on glucose from hatching were transferred to the appropriate experimental plate for scoring.
For oxidative stress tests, worms were grown on NGM or NGM + 2% glucose and transferred to NGM plates + 240 μ m juglone at adult day 1.
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We also need better diagnostic tests; worm infections like cysticercosis and toxocariasis can often be treated effectively with antiparasitics and anti-inflammatories, but they are frequently misdiagnosed.
To distinguish context conditioning from blocking, we tested worms to benzaldehyde (CS2) in the NH4Cl (contextnthey they were trained in.
The biologists trained and tested worms of a variety of ages to assess the cognitive abilities of young, middle-aged and old worms.
Perlstein Lab uses CRISPR, the command line for editing genomes, to test worms, flies, and fish for ways to cure these diseases.
It is clear that at the higher concentrations, Vt.Cr produced paralysis in the test worms.
To test worms in comparable satiation states, worms tested under the 'fed' status were assayed within 10 min of being extracted from their bacterial lawn.
To test worms in the 'starved' state, we allowed worms to remain suspended in liquid Nematode Growth Media NGMM) for 30 min prior to beginning their run.
Your response mentions that you always tested worms in two conditions at the same time, and on the same day with the same reagents.
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