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Tests were incubated at 10°C, 150 rpm, away from light, and run in triplicate.
Tests were incubated aerobically at 37°C for 24 hours before being read.
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Biochemical tests were performed as follows with negative tests being incubated for 7 days before discarding unless otherwise indicated.
Blood collected in serum separator tubes for the remaining tests was incubated on ice for 90 min, and centrifuged at 1600 × g (20 min at 4 °C).
For S. cerevisiae, patches of strains to be tested were incubated on YPD plates or SPO plates and then a sterile toothpick was used to make a patch of the yeast on the agar surface of a 50 mm petri dish containing 10 ml of 2% agar.
To ascertain that the reaction of antibody was specific, sections from each test were incubated with normal serum IgG separately.
Monocytes from the patients under test were incubated in the presence or in the absence of HMGB1, LPS or LPS plus HMGB1.
A mixture of the phenol red solution and the enzymatic suspension being tested was incubated at 37°C for a maximum of 2 hours.
The test tubes were incubated in a stirring incubator at 37°C and stirred at 120 rpm for 24 h.
For susceptibility testing, plates with the antibiotic disks and E-test strips were incubated in 5% CO2.
Test dishes were incubated at a constant temperature of 26 ± 1 °C in a climate-controlled incubator with light conditions of 300 700 lx and a light:dark cycle of 12 12 h.
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