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Any discordant tests were confirmed using Western Blot.
All initially positive tests were confirmed using Immunocomb HIV 1 and 2 kits (Orgenics, Yavne, Israel).
Positive tests were confirmed using a second ELISA (Recombigen, Cambridge Biotech, Worcester, Massachusetts, USA).
Sera tested positive by RPR tests were confirmed using treponemal test, modified Treponema pallidum haemagglutination assay (TPHA) (Syphicheck WB, Qualpro Diagnostics, India).
HIV antibody was detected using Micro ELISA (J. Mitra and Company, India), and positive tests were confirmed using Genedia HIV ELISA 3.0 (Green Cross Life Science Corporation, South Korea).
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Validity of all statistical tests was confirmed using bootstrapped p-values (n = 1,000,000, see Supplementary Tables 1–5).
The CDC recommends that reactive rapid tests be confirmed using an HIV Western blot [2, 29].
The validity of applying parametric tests was confirmed using non-parametric bootstrapping [ 27], as recommended for the analysis of cost data [ 26].
Children under 12 months of age with a positive test were confirmed using PCR.
Mycoplasma-positive PCR test results were confirmed using a detection kit (MycoAlert®; Lonza Rockland, ME) based on enzymatic ATP-conversion combined with luminescence measurement.
Five of seven tested transcripts were confirmed using these methods.
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