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Gene specific confidence limits for the respective paired t tests were calculated by bootstrap analysis.

Probabilities for permuted tests were calculated by asymptotic inference.

The effect sizes of the nonparametric tests were calculated by r = Z/√ n.

The effect sizes of the parametric tests were calculated by r = t/(t+ df).

Likelihood ratio tests were calculated by comparing M1a to M2a with df = 2.

Sensitivity, specificity and predictive values for positive and negative tests were calculated by standard methods.

Accurate recall on the interim and final tests was calculated by dividing the total number of trials in which participants produced correct video details by the total number of trials for that given item type.

The one-sided P value for these tests was calculated by comparing the observed mean difference to the mean differences calculated from 10,000 randomizations of the same sets of relatedness estimates using POPTOOLS 2.6 [65].

The DI from the clamp tests was calculated by the C-peptide iAUC multiplied by the M-value.

The volume of H2 produced in sealed vials during glycerol fermentation tests was calculated by the mass balance equation [ 33].

Diagnostic accuracy of the tests was calculated by comparing the ICT results with the reference (composite IFA and PCR) results.