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miR-208a and miR-208b were not detected in any of the examined samples (in both healthy group of dogs ACVIM stage A and ACVIM stage B).> Using the same statistical tests the expression of miRNAs in ACVIM stage C and unaffected dogs was analysed.
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These results differ from previous studies that tested the expression of these receptors in RE.
To further validate this result, we treated LM2 cells with specific inhibitors for the ERK, p38 and JNK pathways and tested the expression of BCLM-associated genes.
Both Western blot and immunohistochemical staining analyses were used to test the expression of Hpa in HepG2, SGC-7901, MKN45, MCF-7, SW480, and U2OS cells.
We have tested the expression of eight integral membrane pyrophosphatases in Saccharomyces cerevisiae, six from bacterial and archaeal sources and two from protozoa.
In addition to the in silico results showing that BCAL2958 is encoded within all the Bcc genomes examined, we have also tested the expression of the protein by a panel of 12 Bcc strains by western-blot techniques.
We tested the expression of the chromosome 3 OXO genes in Vandana and Moroborekan as well as in their progeny to determine if expression of any member correlated with involvement in quantitative resistance.
To further explore the effect of CBHA on SCNT embryo development, we tested the expression of five important genes at the transcription level in the blastocysts of SCNT embryos, CBHA-SCNT embryos, and IVF embryos.
We then tested the expression of a few other markers by RT-PCR.
When tumors reached to the size of 9×9 mm, we tested the expression of miR-20b in different tumor regions.
Primer sets used to test the expression of the two forms of CTIP2 were designed using Primer3 Software.
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